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densitometry quantification  (Bio-Rad)


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    Bio-Rad densitometry quantification
    Densitometry Quantification, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 36402 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/densitometry+quantifications/Image+Lab+Software/pm41397959-607-0-7
    Average 99 stars, based on 36402 article reviews
    densitometry quantification - by Bioz Stars, 2026-10
    99/100 stars

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    other:

    Article Title: Altered heart rate control in transgenic mice carrying the KCNJ6 gene of the human chromosome 21.
    Article Snippet: Jacques M. Lignon, Zoë Bichler, Bruno Hivert, François E. Gannier, Pierre Cosnay, José A. del Rio, Danièle Migliore-Samour, and Claire O. Malécot Centre National de la Recherche Scientifique Unité Mixte de Recherche 6542, Physiologie des Cellules Cardiaques et Vasculaires, Université François-Rabelais, Parc Grandmont, Tours, France; Department of Cell Biology, Institute for Research in Biomedicine, University of Barcelona, Barcelona, and CIBERNED, Spain; and Centre National de la Recherche Scientifique Institut de Trangénose, Orléans-La Source, France

    Software:

    Article Title: RBFOX2 regulated EYA3 isoforms partner with SIX4 or ZBTB1 to control transcription during myogenesis
    Article Snippet: The PCR products were resolved via 6% polyacrylamide gel electrophoresis in TAE buffer (40 mM Tris, 20 mM acetic acid, 1 mM EDTA) at 140 V for 1-2 h. Gels were incubated for 10 min in an ethidium bromide solution (0.4 μg/mL) at room temperature prior to imaging on the ChemiDocTM XRS+ (Biorad). .. Densitometry quantifications were performed using the Biorad Image Lab software. .. The product-length dependent staining bias of ethidium bromide was corrected using a standard curve based on the pUC19 ladder (Thermo Fisher Scientific, SM0221).

    Article Title: Shoc2 Is Targeted to Late Endosomes and Required for Erk1/2 Activation in EGF-Stimulated Cells
    Article Snippet: .. Several x-ray films were analyzed to determine the linear range of the chemiluminescence signals, and the quantifications were performed using densitometry analysis mode of the QuantityOne software (Bio-Rad, Inc). ..



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    densitometry scan quantification  (Bio-Rad)
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    Bio-Rad densitometry scan quantification
    A, B : Purified human HK (20, 40, 80, and 110ng) and 0.5μL of plasma from five male healthy donors, (H1 to H5) (A), and five females (H6 to H10) (B) were subjected to SDS-PAGE. The signal of HK native chain (N-HK), light chain (LC-HK), and cleaved light chain (cLC-HK) were quantified by <t>densitometry</t> and N-HK concentration was evaluated by the displayed linear regression. C, D : Box-plot displaying 5–95% range ( italic fonts), median ( bold fonts), and interquartile values of plasma concentration of N-HK (C) and HK cleavage (D), measured as in panel A. densito: densitometry in 10 7 arbitrary units; NS: non-significant; ****: p <10 −4 (Mann-Whitney-Wilcoxon test).
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    A, B : Purified human HK (20, 40, 80, and 110ng) and 0.5μL of plasma from five male healthy donors, (H1 to H5) (A), and five females (H6 to H10) (B) were subjected to SDS-PAGE. The signal of HK native chain (N-HK), light chain (LC-HK), and cleaved light chain (cLC-HK) were quantified by densitometry and N-HK concentration was evaluated by the displayed linear regression. C, D : Box-plot displaying 5–95% range ( italic fonts), median ( bold fonts), and interquartile values of plasma concentration of N-HK (C) and HK cleavage (D), measured as in panel A. densito: densitometry in 10 7 arbitrary units; NS: non-significant; ****: p <10 −4 (Mann-Whitney-Wilcoxon test).

    Journal: PLoS ONE

    Article Title: Kininogen Cleavage Assay: Diagnostic Assistance for Kinin-Mediated Angioedema Conditions

    doi: 10.1371/journal.pone.0163958

    Figure Lengend Snippet: A, B : Purified human HK (20, 40, 80, and 110ng) and 0.5μL of plasma from five male healthy donors, (H1 to H5) (A), and five females (H6 to H10) (B) were subjected to SDS-PAGE. The signal of HK native chain (N-HK), light chain (LC-HK), and cleaved light chain (cLC-HK) were quantified by densitometry and N-HK concentration was evaluated by the displayed linear regression. C, D : Box-plot displaying 5–95% range ( italic fonts), median ( bold fonts), and interquartile values of plasma concentration of N-HK (C) and HK cleavage (D), measured as in panel A. densito: densitometry in 10 7 arbitrary units; NS: non-significant; ****: p <10 −4 (Mann-Whitney-Wilcoxon test).

    Article Snippet: Using enhanced chemiluminescence (Clarity Western ECL TM substrate, Biorad, Hercules, USA) and densitometry scan quantification (ChemiDoc TM XRS+ System camera and Image Lab TM software, Bio-Rad, Hercules, USA), three molecular species were retained for data processing: native protein (N-HK, 120kDa), light chain (LC-HK, 56kDa), and cleaved light chain (cLC-HK, 46kDa).

    Techniques: Purification, Clinical Proteomics, SDS Page, Concentration Assay, MANN-WHITNEY

    A : Purified human HK (20, 40, 80, and 110ng) and 0.5μL of plasma from nC1Inh-AE male patients (M1 to M5) were subjected to SDS-PAGE. The signal of HK native chain (N-HK), light chain (LC-HK), and cleaved light chain (cLC-HK) were quantified by densitometry and N-HK concentration by the displayed linear regression. B, C : Box-plot displaying 5–95% range ( italic fonts), median ( bold fonts), and interquartile values of plasma concentration of N-HK (B) and HK cleavage (C), measured as in panel A, compared to the healthy cohort studied in . D, E, F : as in A, B, C, concerning female patients (W1 to W5). densito: densitometry in 10 7 arbitrary units; ****: p <10 −4 (Mann-Whitney-Wilcoxon test); nC1Inh-AE: angioedema with normal C1 inhibitor.

    Journal: PLoS ONE

    Article Title: Kininogen Cleavage Assay: Diagnostic Assistance for Kinin-Mediated Angioedema Conditions

    doi: 10.1371/journal.pone.0163958

    Figure Lengend Snippet: A : Purified human HK (20, 40, 80, and 110ng) and 0.5μL of plasma from nC1Inh-AE male patients (M1 to M5) were subjected to SDS-PAGE. The signal of HK native chain (N-HK), light chain (LC-HK), and cleaved light chain (cLC-HK) were quantified by densitometry and N-HK concentration by the displayed linear regression. B, C : Box-plot displaying 5–95% range ( italic fonts), median ( bold fonts), and interquartile values of plasma concentration of N-HK (B) and HK cleavage (C), measured as in panel A, compared to the healthy cohort studied in . D, E, F : as in A, B, C, concerning female patients (W1 to W5). densito: densitometry in 10 7 arbitrary units; ****: p <10 −4 (Mann-Whitney-Wilcoxon test); nC1Inh-AE: angioedema with normal C1 inhibitor.

    Article Snippet: Using enhanced chemiluminescence (Clarity Western ECL TM substrate, Biorad, Hercules, USA) and densitometry scan quantification (ChemiDoc TM XRS+ System camera and Image Lab TM software, Bio-Rad, Hercules, USA), three molecular species were retained for data processing: native protein (N-HK, 120kDa), light chain (LC-HK, 56kDa), and cleaved light chain (cLC-HK, 46kDa).

    Techniques: Purification, Clinical Proteomics, SDS Page, Concentration Assay, MANN-WHITNEY

    High-molecular-weight kininogen (HK) molecular pattern of plasma samples from healthy women taking or not estrogen/progestin combination (OP; Healthy+OP, n = 32; Healthy-OP, n = 30) and from nC1Inh-AE patients taking OP and ≥2 months after OP withdrawal (nC1INh-AE+/-OP, n = 13). A : Analysis of plasma samples (0.5μL/lane) from six patients (O1–O6) and the corresponding figures. N-HK concentration of P1 and P2 was evaluated by the displayed linear regression, kinin-forming activity was determined as in , pathological values were labelled in bold fonts. B, C : Box-plot displaying 5–95% range ( italic fonts), median ( bold fonts), and interquartile values of the plasma concentration of N-HK (B) and cleaved HK species (C), measured as in panel A. D, E : Details of the paired samples of panel B and C respectively analysed using the Wilcoxon matched-pairs signed rank test. densito: densitometry in 10 7 arbitrary units; NS : non-significant; *: p <0.05; **: p <0.01; ***: p <0.001; ****: p <10 −4 (non-parametric tests); N-HK, LC-HK, cLC-HK: native chain, light chain, cleaved light chain of HK respectively; nC1Inh-AE: angioedema with normal C1 inhibitor.

    Journal: PLoS ONE

    Article Title: Kininogen Cleavage Assay: Diagnostic Assistance for Kinin-Mediated Angioedema Conditions

    doi: 10.1371/journal.pone.0163958

    Figure Lengend Snippet: High-molecular-weight kininogen (HK) molecular pattern of plasma samples from healthy women taking or not estrogen/progestin combination (OP; Healthy+OP, n = 32; Healthy-OP, n = 30) and from nC1Inh-AE patients taking OP and ≥2 months after OP withdrawal (nC1INh-AE+/-OP, n = 13). A : Analysis of plasma samples (0.5μL/lane) from six patients (O1–O6) and the corresponding figures. N-HK concentration of P1 and P2 was evaluated by the displayed linear regression, kinin-forming activity was determined as in , pathological values were labelled in bold fonts. B, C : Box-plot displaying 5–95% range ( italic fonts), median ( bold fonts), and interquartile values of the plasma concentration of N-HK (B) and cleaved HK species (C), measured as in panel A. D, E : Details of the paired samples of panel B and C respectively analysed using the Wilcoxon matched-pairs signed rank test. densito: densitometry in 10 7 arbitrary units; NS : non-significant; *: p <0.05; **: p <0.01; ***: p <0.001; ****: p <10 −4 (non-parametric tests); N-HK, LC-HK, cLC-HK: native chain, light chain, cleaved light chain of HK respectively; nC1Inh-AE: angioedema with normal C1 inhibitor.

    Article Snippet: Using enhanced chemiluminescence (Clarity Western ECL TM substrate, Biorad, Hercules, USA) and densitometry scan quantification (ChemiDoc TM XRS+ System camera and Image Lab TM software, Bio-Rad, Hercules, USA), three molecular species were retained for data processing: native protein (N-HK, 120kDa), light chain (LC-HK, 56kDa), and cleaved light chain (cLC-HK, 46kDa).

    Techniques: High Molecular Weight, Clinical Proteomics, Concentration Assay, Activity Assay

    A : High-molecular-weight kininogen (HK) molecular pattern of plasma samples (0.5μL/lane) from two nC1Inh-AE patients (W6, M6) less than 24h after the onset of an AE attack (Att) and at least two weeks after remission (Rem), analysed as in . B : Same as for A, with plasma samples from three patients (K1–K3) with ACEi-AE and low kinin catabolism (L-Cat) and three patients (M7–M9) with nC1Inh-AE and normal kinin catabolism (N-Cat). N-HK concentrations were evaluated by the displayed linear regressions, kinin-forming activity was determined as in , pathological values were labelled in bold font. densito: densitometry in 10 7 arbitrary units; N-HK, LC-HK, cLC-HK: native chain, light chain, cleaved light chain of HK respectively.

    Journal: PLoS ONE

    Article Title: Kininogen Cleavage Assay: Diagnostic Assistance for Kinin-Mediated Angioedema Conditions

    doi: 10.1371/journal.pone.0163958

    Figure Lengend Snippet: A : High-molecular-weight kininogen (HK) molecular pattern of plasma samples (0.5μL/lane) from two nC1Inh-AE patients (W6, M6) less than 24h after the onset of an AE attack (Att) and at least two weeks after remission (Rem), analysed as in . B : Same as for A, with plasma samples from three patients (K1–K3) with ACEi-AE and low kinin catabolism (L-Cat) and three patients (M7–M9) with nC1Inh-AE and normal kinin catabolism (N-Cat). N-HK concentrations were evaluated by the displayed linear regressions, kinin-forming activity was determined as in , pathological values were labelled in bold font. densito: densitometry in 10 7 arbitrary units; N-HK, LC-HK, cLC-HK: native chain, light chain, cleaved light chain of HK respectively.

    Article Snippet: Using enhanced chemiluminescence (Clarity Western ECL TM substrate, Biorad, Hercules, USA) and densitometry scan quantification (ChemiDoc TM XRS+ System camera and Image Lab TM software, Bio-Rad, Hercules, USA), three molecular species were retained for data processing: native protein (N-HK, 120kDa), light chain (LC-HK, 56kDa), and cleaved light chain (cLC-HK, 46kDa).

    Techniques: High Molecular Weight, Clinical Proteomics, Activity Assay